- 186602 - Cell Biologist
- CA-Southern California - San Diego
- 12 Months
- Chipton-Ross is seeking a Cell Biologist for an opening in Carlsbad, CA.
This scientist will execute service based projects that consist of high-throughput molecular biology and cell line generation activities that use cutting edge cloning and genomic engineering techniques from the Client portfolio. This scientist will be expected to lead some projects independently and to work with other scientists in a highly collaborative team environment. Projects may include, but not be limited to, cloning and DNA preparation, in vitro transcription of RNA, lentiviral production and cell line engineering, with cell engineering being the primary focus; these projects may be driven by internal project design or by external collaborations.
The successful individual will directly interact with senior R&D scientists and project management to execute project workplans. The scientist will be responsible for executing or supporting these plans to meet agreed upon deliverables and timelines. These activities will require a high level of independence as well as the ability to work in a highly cooperative team.
This role will involve the following tasks:
Tissue culture (culture of many cell types)
Stable cell line generation (transfection, transduction, antibiotic-selection, clonal expansion)
Genomic editing (TALN and CRISPR based editing, design and implementation)
Plasmid subcloning, DNA preparations, DNA sequencing, in vitro transcription of RNA, packaging of lentivirus, PCR, RT-PCR, TaqMan® qPCR
Automation of plasmid cloning and molecular biology
This individual will also be expected to support and participate in R&D and project management meetings.
1+ year experience in molecular biology techniques (DNA/RNA)
1+ year experience in sterile tissue culture
This position requires the following capabilities and competencies:
Perform standard laboratory math calculations and solutions/media preparation
Proven sterile tissue culture technique
Proven experience manipulating DNA plasmid constructs and with various forms of PCR
Ability to observe deviations from the expected outcomes
Ability to succeed with a moderate level of supervision and guidance
Strong teamwork orientation with demonstrable influence/interpersonal skills
This position will benefit from:
Proven experience generating stable cell lines
Proven experience with genome engineering of mammalian cells
1+ year experience in stable cell line generation
1+ year experience with genome engineering
The vast majority of working time will be spent in a laboratory environment. Most of the work will take place in biosafety hoods in a tissue culture laboratory, working with various cell cultures. Cells will typically be validated by molecular biology techniques or functional assays. Time may be spent manipulating plasmid DNA constructs in a laboratory environment. Some time will be spent at the desk recording laboratory activities appropriately in notebooks.
Must be capable of observing and adhering to lab safety standards and protocols. Occasional weekend work may be required to maintain cell cultures properly.
Critical to success in this position will be the individual’s ability to independently work on some projects and to provide support on others. This individual must be able to identify deviations from expected outcomes and participate in communicating and trouble-shooting those deviations, and suggesting alternatives. Equally important will be the ability to work as part of a team in the laboratory (extended R&D team) as well as a broader cross-functional team (R&D, Project Management, Collaborator).
Accredited Associate’s degree (required). Candidates with a bachelor’s degree or industry experience (preferred).
Employment will be contingent on clearing a drug screen and background check. Both must clear prior to start date.
420 Culver Boulevard
Playa Del Rey, CA 90293
Phone: (310) 414-7800 x307 or (800) 927-9318 x307
Candidates responding to this posting must currently possess the eligibility to work in the United States. No third parties please.
Employment will be contingent on candidate clearing pre-employment drug screen and background check.
Chipton-Ross provides equal employment opportunities to all employees and applicants for employment without regard to race, color, creed, religion, national origin, sex (including pregnancy), age, disability, sexual orientation, gender identity and/or expression, protected veteran status, genetic information, or any other characteristic protected by Federal, State or local law. This policy governs all areas of employment at Chipton-Ross, including recruiting, hiring, training, assignment, promotions, compensation, benefits, discipline, and terminations.