- 188907 - Scientist II
- CA-Southern California - San Diego
- 12 Months
- Chipton-Ross is seeking a Scientist II for an opening in Carlsbad, CA.
Be part of the genome engineering revolution. This scientist will develop tools and techniques to support genome engineering. This scientist will be expected to work in a highly collaborative team environment, supporting some projects with some supervision of a senior scientist and leading some projects independently. Projects may include, but not be limited to, cloning, developing gene editing strategies, and developing assays to monitor editing.
The successful individual will directly interact with senior R&D scientists and project management to execute project work plans. The scientist will be responsible for supporting or executing these plans to meet agreed upon deliverables and timelines. These activities will require a high level of independence as well as the ability to work in a highly cooperative team.
This role will involve the following tasks:
Mammalian cell culture
DNA delivery and mammalian cell transfection
Genomic editing in mammalian cell lines (TALN and CRISPR based editing, design and implementation)
Flow cytometric analysis
Plasmid subcloning, DNA preparations, sequencing, in vitro transcription of RNA, PCR
This individual will also be expected to support and participate in R&D and project management meetings.
Proven experience in cell biology
mammalian cell culture
Lipid-based cell transfection and electroporation
Cell engineering (CRISPR or TALEN)
Cell-based assays, such as FACS, immunocytochemistry, ELISA, and Western Blotting
Experience in molecular biology
DNA cloning and sequencing
DNA, RNA preparation
PCR, qPCR assays
Observe deviations from the expected outcomes and trouble-shoot
Succeed with a moderate level of supervision and guidance
Participate in a strong teamwork orientation with demonstrable skills
Maintain a detailed lab notebook and present your findings in a group setting
The vast majority of working time will be spent in a laboratory environment. Cell work will take place in biosafety hoods in a tissue culture laboratory, working with various cell cultures. Time may be spent manipulating DNA constructs and proteins in a laboratory environment. Some time will be spent at the desk recording laboratory activities appropriately in notebooks.
Candidate must be capable of observing and adhering to lab safety standards and protocols.
This individual must be able to identify deviations from expected outcomes and participate in troubleshooting those deviations and suggesting alternatives.
Critical to success in this position will be the individual’s ability to work independently on some projects and to provide support collaboratively on others while meeting project timelines.
Equally important will be the ability to work as part of a team in the laboratory (extended R&D team) as well as a broader cross-functional team (R&D, Project Management, Collaborator).
Accredited Bachelor's degree with 3-5 years experience in cell biology, master’s degree with 3+ years of experience in cell biology.
Employment will be contingent on clearing a drug screen and background check. Both must clear prior to start date.
420 Culver Boulevard
Playa Del Rey, CA 90293
Phone: (310) 414-7800 x307 or (800) 927-9318 x307
Candidates responding to this posting must currently possess the eligibility to work in the United States. No third parties please.
Employment will be contingent on candidate clearing pre-employment drug screen and background check.
Chipton-Ross provides equal employment opportunities to all employees and applicants for employment without regard to race, color, creed, religion, national origin, sex (including pregnancy), age, disability, sexual orientation, gender identity and/or expression, protected veteran status, genetic information, or any other characteristic protected by Federal, State or local law. This policy governs all areas of employment at Chipton-Ross, including recruiting, hiring, training, assignment, promotions, compensation, benefits, discipline, and terminations.