189014 - Scientist I
CA-Southern California - San Diego
Up to $29.99 DOE
12 mos
Chipton-Ross is seeking a Scientist I for an opening in Carlsbad, CA.

This scientist will execute service based projects that primarily consist of genome engineering tool generation using the cutting edge cloning and genomic engineering techniques from the client portfolio. This scientist will be expected to support projects and lead projects by leading cloning, sequencing, virus generation, and bio-informatics with an extensive use of automated liquid handling and laboratory information management systems. This scientist will act in a highly collaborative manner with the rest of the team through design, sequencing, analysis and large-scale manipulations of projects. Projects may include, but not be limited to subcloning, plasmid preparations, in vitro transcription of RNA, lentiviral production; these projects may be driven by internal project design or by external collaborations.

The successful individual will directly interact with R&D scientists and project management to execute a large number of specific project workplans. The scientist will be responsible for executing portions of these plans to meet agreed upon deliverables and timelines. These activities will require a high level of independence as well as the ability to work in a highly cooperative team.

This role will involve the following tasks:
Liquid handling:
Experience using liquid handling instrumentation for cloning, sequencing (Sanger and NGS), cell culture and cell-based assays, and molecular biology

Cell Culture of adherent and suspension cell types:
Transfection, transduction, antibiotic-selection, clonal expansion. Flow cytometry.

Molecular Biology:
Plasmid subcloning, DNA preparations, DNA sequencing, in vitro transcription of RNA, packaging of lentivirus, PCR, RT-PCR, TaqMan® qPCR

Genomic Editing:
TALN and CRISPR based editing, design and implementation.

Bio-informatics and Information Management:
Experience using bio-informatic approaches and tools in applied situations. Experience using, designing, maintaining or administering LIMS systems to manage laboratory execution. Specifically, experience with Nautilus LIMS from the company will be a significant plus.

This individual will also be expected to support and participate in R&D and project management meetings. Multi-tasking and maintaining clear records of work performed. Proven sterile tissue culture technique. Use of automation for liquid handling

Ability to:
Observe deviations from the expected outcomes and trouble-shoot. Succeed with a moderate level of supervision and guidance. Strong communication and teamwork orientation with demonstrable influence/interpersonal skills.

This position will benefit from:
Proven experience:
Sequencing DNA on multiple platforms. Flow cytometry. Bioinformatics tools for design and analysis. Using LIMS to support laboratory execution.


Required:1+ year experience in cell culture
Required: experience in automation for liquid handling
Preferred: experience in flow cytometry
Preferred: experience in cloning
Preferred: experience in industry
Preferred: experience DNA sequencing
Preferred: experience with bio-informatics/LIMS

Working Conditions:
The vast majority of working time will be spent in a laboratory environment. Work will be focused around cell culture, designing, ordering, cloning and otherwise manipulating DNA constructs, and generating either lentivirus or in vitro transcribed RNA using automated liquid handling equipment and LIMS. Sequencing will also be critical throughout these tasks, using both capillary electrophoresis and the Ion Personal Genome machine. Proven experience using automated liquid handlers and the use, design or support Nautilus LIMS to execute projects will be a significant plus compared to other candidates. Experience in industry will also be considered a significant differentiator. Some time will be spent at the desk recording laboratory activities appropriately in notebooks. Must be capable of observing and adhering to lab safety standards and protocols. Flexibility in work schedule may be required: Early mornings, late evenings or weekend work may be required to maintain project schedules. Critical to success in this position will be the individual’s ability to work independently on some projects and to provide collaboratively on others. This individual must be able to identify deviations from expected outcomes and participate in trouble-shooting those deviations and suggesting alternatives. Equally important will be the ability to work as part of a team in the laboratory (extended R&D team) as well as a broader cross-functional team (R&D, Project Management, Collaborator).

Accredited Bachelor's Degree.


Employment will be contingent on clearing a drug screen and background check. Both must clear prior to start date.

Tyler Harrell
420 Culver Boulevard
Playa Del Rey, CA 90293

Phone: (310) 414-7800 x307 or (800) 927-9318 x307

Candidates responding to this posting must currently possess the eligibility to work in the United States. No third parties please.

Employment will be contingent on candidate clearing pre-employment drug screen and background check.

Chipton-Ross provides equal employment opportunities to all employees and applicants for employment without regard to race, color, creed, religion, national origin, sex (including pregnancy), age, disability, sexual orientation, gender identity and/or expression, protected veteran status, genetic information, or any other characteristic protected by Federal, State or local law. This policy governs all areas of employment at Chipton-Ross, including recruiting, hiring, training, assignment, promotions, compensation, benefits, discipline, and terminations.